In The Clone Wars, the motif appears in the episode The Academy when Ahsoka arrives on Mandalore and in the last four episodes of the show's fifth season, which deal with Ahsoka Tano's confrontation with and eventual resignation from the Jedi Order.
The leitmotif transitions from "Yoda's Theme," which shares its opening notes with Ahsoka's Theme, in the Forces of Destiny episode "Teach You, I Will," where Yoda trains Ahsoka Tano to stay true to herself.
Ahsoka's Theme played as Garazeb Orrelios let go of Ezra Bridger aboard an Imperial cruiser in Star Wars Rebels: Spark of Rebellion, leaving Bridger to be captured by Alexsandr Kallus.